1. T.H. Segall-Shapiro, E. D. Sontag, and C. A. Voigt. Engineered promoters enable constant gene expression at any copy number in bacteria. Nature Biotechnology, 36:352-358, 2018. [PDF] Keyword(s): synthetic biology, systems biology, genetic circuits, gene copy number, incoherent feedforward loop, feedforward, IFFL. Abstract:

   This paper deals with the design of promoters that maintain constant levels of expression, whether they are carried at single copy in the genome or on high-copy plasmids. The design is based on an incoherent feedforward loop (iFFL) with a perfectly non-cooperative repression. The circuits are implemented in E. coli using Transcription Activator Like Effectors (TALEs). The resulting stabilized promoters generate near identical expression across different genome locations and plasmid backbones (pSC101, p15a, ColE1, pUC), and also provide robustness to strain mutations and growth media. Further, their strength is tunable and can be used to maintain constant ratios between proteins.




2. T.H. Segall-Shapiro, A.J. Meyer, A.D. Ellington, E.D. Sontag, and C.A. Voigt. A `resource allocator' for transcription based on a highly fragmented T7 RNA polymerase. Molecular Systems Biology, 10:742-, 2014. [WWW] [PDF] Keyword(s): systems biology, synthetic biology, gene expression. Abstract:

   A transcriptional system is built based on a 'resource allocator' that sets a core RNAP concentration, which is then shared by multiple sigma fragments, which provide specificity. Adjusting the concentration of the core sets the maximum transcriptional capacity available to a synthetic system.

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